doi: 10.1055/s-0028-1093595.
Superior immunoreactivity of 125I (Des-Tyr-betaAla)-secretin with rabbit anti-secretin sera compared to 125I-secretin and 125 I-6-Tyrosyl secretin
- PMID: 1002105
- DOI: 10.1055/s-0028-1093595
Item in Clipboard
Superior immunoreactivity of 125I (Des-Tyr-betaAla)-secretin with rabbit anti-secretin sera compared to 125I-secretin and 125 I-6-Tyrosyl secretin
Horm Metab Res.
1976 Nov.
Abstract
A secretin analogue in which the normal amino acid sequence had been elongated by a (Des-Tyr-betaAla)-residue was studied as tracer for secretin radioimmunoassay. 125I-(DATA)-secretin exhibited superior immunoreactivity with several rabbit anti-secretin sera compared to 125I-6-Tyr-secretin and also to secretin iodinated at its N-terminal histidyl residue. This may be due, at least in part, to higher conformational integrity of the secretin moiety in the 125I-(DATA)-secretin molecule. Thus, at present, 125I-(DATA)-secretin appears to be most suitable as tracer for sensitive secretin radioimmunoassay.
Similar articles
-
Secretin radioimmunoassay using 125I-6-tyrosyl-secretin (6TS).Horm Metab Res. 1975 Sep;7(5):447. doi: 10.1055/s-0028-1095678. Horm Metab Res. 1975. PMID: 1183929 No abstract available.
-
Comparative reactivities of 125I-secretin and 125I-minus6-tyrosyl secretin with guinea pig and rabbit anti-secretin sera.Biochem Biophys Res Commun. 1975 Jan 2;64(3):1036-40. doi: 10.1016/0006-291x(75)90151-5. Biochem Biophys Res Commun. 1975. PMID: 1170858 No abstract available.
-
Immunological aspects of secretin, substance P, and VIP.Gastroenterology. 1977 Apr;72(4 Pt.2):803-10. Gastroenterology. 1977. PMID: 838240
-
The role of some polar amino acid residues in secretin.Gastroenterology. 1977 Apr;72(4 Pt.2):801-2. Gastroenterology. 1977. PMID: 838239
-
Structural basis of natural ligand binding and activation of the Class II G-protein-coupled secretin receptor.Biochem Soc Trans. 2007 Aug;35(Pt 4):709-12. doi: 10.1042/BST0350709. Biochem Soc Trans. 2007. PMID: 17635130 Review.
Cited by
-
Characterization of secretin release in response to food and intraduodenal administration of fat and hydrochloric acid.Dig Dis Sci. 1991 Apr;36(4):513-9. doi: 10.1007/BF01298885. Dig Dis Sci. 1991. PMID: 2007369
-
Radioimmunoassay of gastrointestinal hormones.World J Surg. 1979 Aug 31;3(4):423-31. doi: 10.1007/BF01556101. World J Surg. 1979. PMID: 516754 No abstract available.
-
Radioimmunoassay of secretin. A critical review and current status.Dig Dis Sci. 1980 Jul;25(7):529-52. doi: 10.1007/BF01315215. Dig Dis Sci. 1980. PMID: 6104568 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
