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. 1976 Aug;80(2):367-70.
doi: 10.1093/oxfordjournals.jbchem.a131285.

Effective method for activity assay of lipase from Chromobacterium viscosum

Y Horiuti, H Koga, S Gocho
Free article

Effective method for activity assay of lipase from Chromobacterium viscosum

Y Horiuti et al. J Biochem. 1976 Aug.
Free article

Abstract

A method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, EC 3.1.1.3] excreted from Chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of Adekatol 45-S-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10--16 carbon atoms) was used as the substrate. This method was specifically effective for Chromobacterium lipase acitvity assay, and was approximately twice as sensitive as the conventional method, in which polyvinyl alcohol is used for the emulsification of the substrate.

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