Separation of arginase isoenzymes from human tissues by agar gel electrophoresis
- PMID: 1003114
Item in Clipboard
Separation of arginase isoenzymes from human tissues by agar gel electrophoresis
J Clin Chem Clin Biochem.
1976 Nov.
Abstract
Arginase (EC 3.5.3.1) from human liver. kidney, mammary gland, and erythrocytes, was separated by agar-gel electrophoresis using barbital buffer pH 8.6. Three isoenzymes were separated. Two of these, A2 and A3, occur in liver and erythrocytes. The same two isoenzymes were found in the kidney, but in reversed proportions. In addition to the A3 isoenzyme, the mammary gland contains a fast anodically moving A1 isoenzyme. The three isoenzymes differ in their degree of sensitivity to ornithine.
Similar articles
-
Microheterogeneity of molecular forms of arginase in mammalian tissues.Indian J Biochem Biophys. 2003 Dec;40(6):400-8. Indian J Biochem Biophys. 2003. PMID: 22900367
-
Different species of arginase in animal tissues.Enzyme. 1973;15(1):198-209. Enzyme. 1973. PMID: 4593959 Review. No abstract available.
-
Oligomeric structure of A1 arginase from rat liver and A4 from kidney. Difference in charge of subunits.Acta Biochim Pol. 1976;23(2-3):151-63. Acta Biochim Pol. 1976. PMID: 823742
-
Purification of rat kidney arginases A1 and A4 and their subcellular distribution.Acta Biochim Pol. 1983;30(1):83-92. Acta Biochim Pol. 1983. PMID: 6408859
-
Isoenzymes.Adv Clin Chem. 1967;9:69-163. doi: 10.1016/s0065-2423(08)60286-3. Adv Clin Chem. 1967. PMID: 5337437 Review. No abstract available.
Cited by
-
Immunohistochemical localisation of arginase in human liver using monoclonal antibodies against human liver arginase.Histochemistry. 1987;87(5):465-70. doi: 10.1007/BF00496818. Histochemistry. 1987. PMID: 3323144
-
2-aminoimidazole amino acids as inhibitors of the binuclear manganese metalloenzyme human arginase I.J Med Chem. 2010 May 27;53(10):4266-76. doi: 10.1021/jm100306a. J Med Chem. 2010. PMID: 20441173 Free PMC article.
-
Binding of α,α-disubstituted amino acids to arginase suggests new avenues for inhibitor design.J Med Chem. 2011 Aug 11;54(15):5432-43. doi: 10.1021/jm200443b. Epub 2011 Jul 18. J Med Chem. 2011. PMID: 21728378 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Research Materials