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. 1976 Aug 2;146(3):299-302.
doi: 10.1007/BF00701254.

Heat-sensitive DNA-binding activity of the cI product of bacteriophage lambda

N C Mandal, M Lieb

Heat-sensitive DNA-binding activity of the cI product of bacteriophage lambda

N C Mandal et al. Mol Gen Genet. .

Abstract

The binding of lambda gene cI product to lambda DNA was studied at temperatures from 0 degrees C to 46 degrees C. Binding activity of the products of cIts mutants was higher at 22 degrees C than at 0 degrees C, 26 degrees C or 30 degrees C. Both cI+ and cIts products lost DNA-binding activity at 46 degrees C, but after subsequent cooling to 22 degrees C, they regained 50-100% of their activity.

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References

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