Some properties of rat liver mitochondrial RNA polymerase
- PMID: 1004501
- DOI: 10.1007/BF01731777
Some properties of rat liver mitochondrial RNA polymerase
Abstract
A rapid method suitable for purifying large amounts of mitochondria from rat liver using isopycnic zonal centrifugation is described. The RNA polymerase isolated from the purified mitochrondria was found associated with one peak when resolved by DEAE Sephadex chromatography. The enzyme was next fractionated on a phosphocellulose column followed by glycerol gradient centrifugation. A 600-fold purification was achieved when the enzyme was finally filtered through agarose gel. This final enzyme fraction consisted of one polypeptide chain as shown by polyacrylamide gel electrophoresis profiles. The enzyme has a greater preference for poly [d(A-T)] templates than for rat liver mitochondrial DNA. Inhibition of the enzyme activity required high concentrations of the inhibitors. The resistance of the enzyme to alpha-amanitin indicated that there was no contamination from nuclear RNA polymerase II. The conclusion is drawn that the mitochondrial RAN polymerase activity is associated with a single polypeptide.
Similar articles
-
Purification and characterization of RNA polymerase I from a higher plant.Biochim Biophys Acta. 1976 Feb 5;418(3):344-57. doi: 10.1016/0005-2787(76)90296-3. Biochim Biophys Acta. 1976. PMID: 1247549
-
Yeast mitochondrial RNA polymerase. Purification and properties of the catalytic subunit.J Biol Chem. 1986 Aug 5;261(22):10340-7. J Biol Chem. 1986. PMID: 3525543
-
Purification and properties of ribonucleic acid polymerase from rat liver mitochondria.Biochemistry. 1973 Dec 4;12(25):5096-101. doi: 10.1021/bi00749a012. Biochemistry. 1973. PMID: 4792297 No abstract available.
-
Demonstration of RNA polymerase multiplicity in Trypanosoma brucei. Characterization and purification of alpha-amanitin-resistant and -sensitive enzymes.Biochem J. 1987 Feb 1;241(3):649-55. doi: 10.1042/bj2410649. Biochem J. 1987. PMID: 3593214 Free PMC article.
-
Properties and subunit composition of RNA polymerase II from plant cell cultures.Biochim Biophys Acta. 1975 Jul 7;395(3):337-46. doi: 10.1016/0005-2787(75)90205-1. Biochim Biophys Acta. 1975. PMID: 1148241
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous