A restriction endonuclease from Staphylococcus aureus
- PMID: 1005115
- PMCID: PMC343162
- DOI: 10.1093/nar/3.11.3193
A restriction endonuclease from Staphylococcus aureus
Abstract
A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.
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