Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1999 Feb 12;64(4):333-41.
doi: 10.1016/s0378-1135(98)00243-0.

Cytotoxic T-cell, delayed type hypersensitive and listeriolysin O responses in experimental bovine listeriosis

Affiliations

Cytotoxic T-cell, delayed type hypersensitive and listeriolysin O responses in experimental bovine listeriosis

S B Barbuddhe et al. Vet Microbiol. .

Abstract

Five different antigens of Listeria monocytogenes NCTC 11994 were assayed for their lymphoproliferative, cytotoxic, humoral and delayed type hypersensitivity (DTH) responses in experimentally infected calves. The antigens used were culture filtrate antigen (CFA), listeriolysin O (LLO), sonicated antigen (SA), live antigen (LA) and heat killed antigen (HKA). CFA was most lymphoproliferative when assayed in vitro. In an autologous monocyte cytotoxicity assay, soluble protein as well as bacterial cells elicited cytolytic responses, however, LA and LLO primed monocytes showed a higher cytotoxic effect. The expression of cell surface markers of lymphocyte subpopulations was almost identical in experimentally infected as well as non-infected calves. SA elicited the highest humoral and DTH responses. LLO being a major virulence factor with appreciable humoral as well as cellular responses may be used as a candidate antigen to develop a reliable diagnostic immunoassay.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources