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. 1999 Apr;37(4):1197-9.
doi: 10.1128/JCM.37.4.1197-1199.1999.

rpoB mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy

G Pozzi  1 , M Meloni, E Iona, G Orrù, O F Thoresen, M L Ricci, M R Oggioni, L Fattorini, G Orefici
Affiliations

rpoB mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy

G Pozzi et al. J Clin Microbiol. 1999 Apr.

Abstract

Mutations of rpoB associated with rifampin resistance were studied in 37 multidrug-resistant (MDR) clinical strains of Mycobacterium tuberculosis isolated in Italy. At least one mutated codon was found in each MDR strain. It was always a single-base substitution leading to an amino acid change. Nine different rpoB alleles, three of which had not been reported before, were found. The relative frequencies of specific mutations in this sample were different from those previously reported from different geographical areas, since 22 strains (59.5%) carried the mutated codon TTG in position 531 (Ser-->Leu) and 11 (29.7%) had GAC in position 526 (His-->Asp).

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Figures

FIG. 1
FIG. 1
Comparison of the relative frequencies of rpoB mutations in RFM-resistant M. tuberculosis strains from different countries. The mutated codons TTG in position 531 (A) and GAC in position 526 (B) represent the two most frequent mutations in Italian strains. Data reported here are from the present study (for Italy) and from the literature for Germany (15), Greece (7), Sierra Leone (15), Japan (12, 13, 16), the United States (3, 6, 8, 11), Mozambique (2), and “different sources” a (9), b (17), c (19), and d (4). The number of strains in which the mutation was found/total number of strains studied is given in each bar.
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