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. 1999 Apr;37(4):1221-3.
doi: 10.1128/JCM.37.4.1221-1223.1999.

Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis

L E Fenelon  1 , A J Hamilton, J I Figueroa, M A Bartholomew, M H Allen, P McCarthy, R J Hay
Affiliations

Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis

L E Fenelon et al. J Clin Microbiol. 1999 Apr.

Abstract

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.

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Figures

FIG. 1
FIG. 1
Immunofluorescence and immunoperoxidase reactivities of MAb 611F in paraffin wax-embedded tissue. (a) Immunofluorescence staining of paraffin wax-embedded section of lung tissue from a patient with invasive aspergillosis caused by A. fumigatus. Representative hyphal elements are indicated by arrows. Bar represents 30 μm. (b) Immunoperoxidase staining of paraffin wax-embedded section of liver from a patient with invasive aspergillosis caused by A. fumigatus. Representative hyphal elements are indicated by arrows. Bar represents 25 μm.
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