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. 1999 Apr;37(4):1224-6.
doi: 10.1128/JCM.37.4.1224-1226.1999.

Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complex

W A Githui  1 , S M Wilson, F A Drobniewski
Affiliations

Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complex

W A Githui et al. J Clin Microbiol. 1999 Apr.

Abstract

Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberculosis complex isolates.

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Figures

FIG. 1
FIG. 1
Isolates of mycobacterial and nonmycobacterial species hybridized with the international INS1/INS2 IS6110 probe. Lane(s): M, Lambda HindIII molecular weight (size) marker; 1 and 12, M. tuberculosis Mt. 14323; 2, M. avium complex; 3, M. xenopi; 4, M. kansasii; 5, M. gordonae; 6, M. fortuitum; 7, M. chelonae; 8, M. malmoense; 9, Nocardia sp.; 10, M. marinum; and 11, E. coli NCTC 10418.
FIG. 2
FIG. 2
Amplified products of various mycobacterial and nonmycobacterial species obtained by using IS6110 two-tube nested PCR. Lane(s): M, 100-bp ladder (Promega, Madison, Wis.); 1, M. avium complex; 2, M. xenopi; 3, M. kansasii; 4, M. gordonae; 5, M. fortuitum; 6, M. chelonae; 7, M. malmoense; 8, Nocardia sp.; 9, M. marinum; 10, E. coli NCTC 10418; 12 and 13, M. tuberculosis Mt. 14323; 15 through 21, negative controls; 23 and 24, BCG strain (Glaxo) positive controls (amplification of 0.05 and 0.5 pg of BCG DNA, respectively); 11, 14 and 22, empty. Arrows indicate the position of a 181-bp IS6110 fragment.
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