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Review
. 1999 Mar;103(6):767-72.
doi: 10.1172/JCI6509.

PDZ domains: fundamental building blocks in the organization of protein complexes at the plasma membrane

A S Fanning  1 , J M Anderson
Affiliations
Review

PDZ domains: fundamental building blocks in the organization of protein complexes at the plasma membrane

A S Fanning et al. J Clin Invest. 1999 Mar.
No abstract available

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Figures

Figure 1
Figure 1
Schematic diagram of a vertebrate polarized epithelial cell, showing the distinct subcellular localization of several epithelial PDZ proteins into the apical, basal-lateral, and junctional domains.
Figure 2
Figure 2
Structural diagrams of the PDZ3 domain of PSD95, depicting interactions with a peptide ligand. (a) Ribbon diagram of the PDZ3 domain. The peptide (yellow) lies in the groove created by the βB strand and the αB helix, and the COOH-terminus of the peptide lies in the hydrophobic activity created by the βA–βB or carboxylate loop. (b) Chemical interactions involved in peptide binding. Stereoscopic images depicting hydrogen bonding (dashed white lines) between residues of the PDZ domains (blue) and the peptide ligand (orange). Oxygen atoms are shown in red and nitrogen atoms in blue. The green sphere is a well-ordered water molecule linking the carboxylate group to Arg318. Reproduced, with permission, from ref. .
Figure 3
Figure 3
Schematic diagram of InaD and NHERF, illustrating the protein–protein interactions mediated by these two PDZ proteins. (a) InaD binds to the G protein (Gqα)–linked receptor rhodopsin (Rh), the light-activated cation channels TRP and TRPL, phospholipase C-β (PLC), and protein kinase C (PKC) via PDZ domains. Calmodulin binds to the region between PDZ1 and PDZ2. A higher-order structure is organized at the plasma membrane by homomeric and heteromeric binding between PDZ domains 3 and 4. Reproduced, with permission, from ref. . (b) The β2-adrenergic receptor binds to the first PDZ domain of NHERF, and the Na+/H+ exchanger NHE-3 binds to a region adjacent to and including the second PDZ domain. Ezrin binds to a domain in the COOH-terminus of NHERF, linking the protein complex to the actin cytoskeleton and protein kinase A (PKA-II). Both PKA-II and the β2-adrenergic receptor regulate the activity of NHE-3.
See this image and copyright information in PMC

References

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