Antibiotics and light responses in superfused bovine retina

Abstract

1. Our objective was to study effects of clindamycin and ciprofloxacin on the electroretinogram (ERG) of isolated bovine retinas. 2. Electroretinograms of isolated superfused bovine retinas were recorded under normal conditions and during application of clindamycin and ciprofloxacin. The b-wave reduction was plotted against the drug concentration. In several cases retinal oxygen uptake was also measured. Clindamycin was available only in a preparation containing benzyl alcohol. To differentiate between effects caused by the antibiotic and the alcohol, ERGs were also recorded under superfusion with benzyl alcohol. To record drug effects on photoreceptors synaptic transmission was blocked using 1 mM aspartate. 3. At concentrations between 0.3 and 10 mM clindamycin significantly reduced the amplitude of the b-wave of the ERG. A comparable reduction of retinal oxygen uptake was found at concentrations 10-fold higher. Clindamycin, 3 mM, did not affect the a-wave after preincubation with aspartate. Benzyl alcohol at concentrations of 0.3 and 1 mM did not affect the b-wave, whereas at higher concentrations the b-wave was found to be reduced. Considerable b-wave reductions were found with ciprofloxacin at concentrations of between 0.03 and 0.6 mM. All effects proved to be fully reversible and dose-dependent. 4. Ciprofloxacin and clindamycin did both alter neural function in the isolated superfused bovine retina. The nontoxic dosages found here differ considerably from results in rabbits after intravitreal injections. This is probably due to species differences.