doi: 10.1016/s0378-4347(98)00542-8.
Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography-mass spectrometry
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- PMID: 10080656
- DOI: 10.1016/s0378-4347(98)00542-8
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Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography-mass spectrometry
J Chromatogr B Biomed Sci Appl.
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Abstract
Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography-mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen-polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories.
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