Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Dec;89(12):1257-66.
doi: 10.1111/j.1349-7006.1998.tb00522.x.

Growth inhibition, enhancement of intercellular adhesion, and increased expression of carcinoembryonic antigen by overexpression of phosphoinositides-specific phospholipase C beta 1 in LS174T human colon adenocarcinoma cell line

K Nomoto  1 , N Tomita, M Miyake, D B Xhu, P R LoGerfo, I B Weinstein
Affiliations

Growth inhibition, enhancement of intercellular adhesion, and increased expression of carcinoembryonic antigen by overexpression of phosphoinositides-specific phospholipase C beta 1 in LS174T human colon adenocarcinoma cell line

K Nomoto et al. Jpn J Cancer Res. 1998 Dec.

Abstract

By using a retrovirus-derived system we generated derivatives of the human colon adenocarcinoma cell line LS174T (ATCC CL 188) that stably overexpress a full-length cDNA encoding the beta 1 isoform of bovine phosphoinositides-specific phospholipase C (PI-PLC). This was confirmed by the elevated levels of catalytic activity to release phosphoinositides from phosphatidylinositol (PI-PLC) or phosphatidylinositol-bis-phosphate (PIP2-PLC), and the enhanced expressions of messenger RNA and protein. PI-PLC beta 1 overexpresser clones grew to form cell clumps floating in liquid medium, whereas the pMV7-introduced control clones displayed morphologic characteristics that were very similar to those of the parent LS174T cell line. Three individual PI-PLC beta 1 overexpresser cell lines displayed increased doubling time (18.0 h, 21.5 h, and 23.8 h) when compared with 4 individual pMV7-introduced control cell lines (13.1 h, 10.7 h, 12.9 h, and 9.3 h). Anchorage-independent growth ability in soft agar medium was dramatically suppressed by overexpression of PLC beta 1, and the ability of PLC-overproducer clones to form aggregates when cultured in liquid medium was dramatically enhanced when compared with that of pMV7-introduced control clones. Tumorigenicity of PLC beta 1-overproducers was much weaker than that of vector-transduced control clones. The spontaneous release of carcinoembryonic antigen from PLC beta 1-overproducer clones was much higher than that from pMV7 control clones. The ability of PLC beta 1-overproducer clones to form aggregates during suspension culture was much stronger than that of the control clones. These results provide the first evidence that elevated levels of endogenous PI-PLC beta 1 suppress tumor cell growth, but enhance the ability to form cell aggregates and to release carcinoembryonic antigen, an intercellular adhesion molecule.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. ) Nishizuka , Y.Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C . Science , 258 , 607 – 614 ( 1992. ). - PubMed
    1. ) Berridge , M. J.Inositol triphosphate and calcium signaling . Nature , 361 , 315 – 325 ( 1993. ). - PubMed
    1. ) Rhee , S. G. and Choi , K. D.Regulation of inositol phospholipid‐specific phospholipase C isozymes . J. Biol. Chem. , 267 , 12393 – 12396 ( 1992. ). - PubMed
    1. ) Lee , C.‐W. , Park , D. J. , Lee , K.‐H. , Kim , C. G. and Rhee , S. G.Purification, molecular cloning, and sequencing of phospholipase C‐β4 . J. Biol. Chem. , 268 , 21318 – 21327 ( 1993. ). - PubMed
    1. ) Rhee , S. G. and Bae , Y. S.Regulation of phosphoinositide‐specific phospholipase C isozymes . J. Biol. Chem. , 272 , 15045 – 15048 ( 1997. ). - PubMed

MeSH terms

Substances

LinkOut - more resources