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. 1999 Mar;15(1):1-4.
doi: 10.1016/s1050-3862(98)00012-6.

A polymerase chain reaction-based screening method for transgenic Arabidopsis

V De Neef  1 , W Van Caeneghem, M Marichal, M Van Montagu, D Van der Straeten
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A polymerase chain reaction-based screening method for transgenic Arabidopsis

V De Neef et al. Genet Anal. 1999 Mar.

Abstract

A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.

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