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. 1999 Jan;55(Pt 1):326-8.
doi: 10.1107/S0907444998004922. Epub 1999 Jan 1.

Preliminary characterization of crystals of an in vitro evolved cyanocobalamin (vitamin B12) binding RNA

D Sussman  1 , D Greensides, K Reilly, C Wilson
Affiliations

Preliminary characterization of crystals of an in vitro evolved cyanocobalamin (vitamin B12) binding RNA

D Sussman et al. Acta Crystallogr D Biol Crystallogr. 1999 Jan.

Abstract

A 35-nucleotide pseudoknot that binds vitamin B12 been isolated using systematic evolution of ligands by exponential enrichment (SELEX). Affinity chromatography was used to purify functional, properly folded molecules and the hanging-drop vapor-diffusion method was used to crystallize this aptamer. Two crystal forms have been obtained and the preliminary crystallographic characterization is reported here. Both crystal forms (space groups I222 or I212121 and C2221) diffract to 2.9 A and should prove sufficient for structure determination.

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