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. 1976 Dec 20;450(3):429-40.
doi: 10.1016/0005-2760(76)90016-3.

The subcellular distribution of carotenoids in Phycomyces blakesleeanus C115 car-42 mad-107 (--)

The subcellular distribution of carotenoids in Phycomyces blakesleeanus C115 car-42 mad-107 (--)

G J Ripley et al. Biochim Biophys Acta. .

Abstract

1. Subcellular fractions of the C115 car-42 mad-107(--) strain of Phycomyces blakesleeanus, prepared by differential centrifugation, were irreversibly associated with extraneous lipids. Such contamination was avoided by the use of density gradient centrifugation. 2. The two beta-carotene-containing fractions, a particulate fraction and lipid globules, also contained sterol, phospholipid and alkaline p-nitrophenylphosphatase activity. Both fractions revealed close similarities on amino acid analysis.

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