Oocyte cryopreservation
- PMID: 10091061
- DOI: 10.1093/humrep/13.suppl_4.98
Oocyte cryopreservation
Abstract
Cryopreservation of human oocytes has been employed with little success in clinical practice, even though it may solve the legal and ethical problems linked to embryo freezing. Various attempts to cryopreserve human oocytes have mostly been unsuccessful, leading to low oocyte survival rates after thawing, and the search for an optimal protocol for oocyte cryopreservation remains elusive. A preliminary study was undertaken to evaluate some of the factors influencing the survival rate of human oocytes and the efficiency of intracytoplasmic sperm injection (ICSI) as an insemination procedure. A total of 38 women with tubal infertility were enrolled in the study. The cryopreservation procedure consisted of a slow freeze-rapid thawing technique using 1,2 propanediol and sucrose as cryoprotectants. The overall oocyte survival rate was approximately 60%. A better survival rate was obtained when the oocytes were cryopreserved in the presence of partially removed cumulus oophorus rather than in the presence of totally enzymatically removed cumulus oophorus. The cryoprotectant concentration and the equilibration time also appear to influence the oocyte survival rate. ICSI may be an efficient method of achieving a satisfactory outcome in terms of fertilization in cryopreserved human oocytes. Embryonic morphological quality does not seem to be compromised by cryopreservation. In conclusion, these data show that cryopreservation may ensure that the integrity of the human oocyte is adequate for normal fertilization and embryo development.
Similar articles
-
Human oocyte cryopreservation: new perspectives regarding oocyte survival.Hum Reprod. 2001 Mar;16(3):411-6. doi: 10.1093/humrep/16.3.411. Hum Reprod. 2001. PMID: 11228204 Clinical Trial.
-
Cryopreservation of human oocytes and ovarian tissue.Cell Tissue Bank. 2006;7(2):113-22. doi: 10.1007/s10561-005-1969-7. Cell Tissue Bank. 2006. PMID: 16732414 Review.
-
Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration.Hum Reprod. 2008 Aug;23(8):1771-7. doi: 10.1093/humrep/den119. Epub 2008 May 12. Hum Reprod. 2008. PMID: 18477574
-
Survival of human oocytes cryopreserved with or without the cumulus in 1,2-propanediol.J Assist Reprod Genet. 1992 Aug;9(4):323-7. doi: 10.1007/BF01203954. J Assist Reprod Genet. 1992. PMID: 1472809
-
Cryopreservation in human assisted reproduction is now routine for embryos but remains a research procedure for oocytes.Hum Reprod. 1998 Jun;13 Suppl 3:161-74;discussion175-7. doi: 10.1093/humrep/13.suppl_3.161. Hum Reprod. 1998. PMID: 9755422 Review.
Cited by
-
Development of new method and protocol for cryopreservation related to embryo and oocytes freezing in terms of fertilization rate: A comparative study including review of literature.Adv Biomed Res. 2016 Jul 6;5:117. doi: 10.4103/2277-9175.185576. eCollection 2016. Adv Biomed Res. 2016. PMID: 27512686 Free PMC article.
-
Successful Pregnancies, Births, and Children Development Following Oocyte Cryostorage in Female Cancer Patients During 25 Years of Fertility Preservation.Cancers (Basel). 2022 Mar 10;14(6):1429. doi: 10.3390/cancers14061429. Cancers (Basel). 2022. PMID: 35326578 Free PMC article.
-
Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin.Molecules. 2022 May 19;27(10):3254. doi: 10.3390/molecules27103254. Molecules. 2022. PMID: 35630729 Free PMC article. Review.
-
Vitrification versus slow freezing for women undergoing oocyte cryopreservation.Cochrane Database Syst Rev. 2014 Sep 5;2014(9):CD010047. doi: 10.1002/14651858.CD010047.pub2. Cochrane Database Syst Rev. 2014. PMID: 25192224 Free PMC article.
-
Human oocyte and ovarian tissue cryopreservation and its application.J Assist Reprod Genet. 2008 Jul;25(7):287-96. doi: 10.1007/s10815-008-9236-z. Epub 2008 Aug 1. J Assist Reprod Genet. 2008. PMID: 18670872 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources