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. 1999 Feb 8;9(3):395-400.
doi: 10.1016/s0960-894x(99)00011-6.

Proof that the ACTVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin

K Ichinose  1 , C Surti, T Taguchi, F Malpartida, K I Booker-Milburn, G R Stephenson, Y Ebizuka, D A Hopwood
Affiliations

Proof that the ACTVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin

K Ichinose et al. Bioorg Med Chem Lett. .

Abstract

Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate, (S)-DNPA, suggesting that the actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.

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