Feedback control of gonadotropins in Atlantic salmon, Salmo salar, male parr.I. Castration effects in rematuring and nonrematuring fish

Abstract

Sexual maturation of Atlantic salmon (Salmo salar) male parr is a seasonally recurrent "all or none" response; either a fish matures fully or it does not mature. To study whether gonadal feedback on gonadotropic hormones, GTH I and GTH II, is involved in the control of maturation, previously mature Atlantic salmon male parr were either sham-operated or castrated in spring. They were then sampled during the onset of gonadal growth (late June-early July) or shortly before the breeding season (late September). In autumn, sham-operated males separated into two groups: nonrematuring males with low pituitary and plasma levels of both GTH I and GTH II, and those rematuring with high levels of gonadotropins. Castrated males had low GTH I and GTH II plasma and pituitary levels, similar to those of the nonrematuring fish, suggesting positive feedback mechanisms, separating the sham-operated fish into low and high GTH level groups. In the summer, plasma GTH II was nondetectable in all fish. Pituitary GTH II content was lower in nonrematuring, than in rematuring males and was even lower in castrated fish. In contrast, castration increased pituitary and plasma levels of GTH I in the summer, suggesting a negative feedback at this reproductive stage. There were no significant differences in immunoassayable levels of GTH I in plasma in rematuring and nonrematuring sham-operated males at this time. The control of rematuration is complex and may involve factors other than circulating GTH I levels, possibly with differences in gonadal sensitivity to GTH I.