Comparative Study
doi: 10.1016/s0167-4781(99)00024-x.
The Bacillus stearothermophilus replicative helicase: cloning, overexpression and activity
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- PMID: 10095066
- DOI: 10.1016/s0167-4781(99)00024-x
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Comparative Study
The Bacillus stearothermophilus replicative helicase: cloning, overexpression and activity
Biochim Biophys Acta.
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Abstract
As part of biochemical and structural studies of the primosome of a gram positive bacterial species, we describe the cloning of the Bacillus stearothermophilus replicative helicase, DnaB. The protein is 45% and 82% identical to the Escherichia coli and B. subtilis replicative helicases, respectively. Recombinant DnaB was purified and shown to be an active helicase.
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