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Comparative Study
. 1999 Mar 19;1444(3):424-8.
doi: 10.1016/s0167-4781(99)00024-x.

The Bacillus stearothermophilus replicative helicase: cloning, overexpression and activity

L E Bird  1 , D B Wigley
Affiliations
Comparative Study

The Bacillus stearothermophilus replicative helicase: cloning, overexpression and activity

L E Bird et al. Biochim Biophys Acta. .

Abstract

As part of biochemical and structural studies of the primosome of a gram positive bacterial species, we describe the cloning of the Bacillus stearothermophilus replicative helicase, DnaB. The protein is 45% and 82% identical to the Escherichia coli and B. subtilis replicative helicases, respectively. Recombinant DnaB was purified and shown to be an active helicase.

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