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. 1999 Apr;43(4):846-9.
doi: 10.1128/AAC.43.4.846.

Molecular characterization of an antibiotic resistance gene cluster of Salmonella typhimurium DT104

C E Briggs  1 , P M Fratamico
Affiliations

Molecular characterization of an antibiotic resistance gene cluster of Salmonella typhimurium DT104

C E Briggs et al. Antimicrob Agents Chemother. 1999 Apr.

Abstract

Salmonella typhimurium phage type DT104 has become an important emerging pathogen. Isolates of this phage type often possess resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT resistance). The mechanism by which DT104 has accumulated resistance genes is of interest, since these genes interfere with treatment of DT104 infections and might be horizontally transferred to other bacteria, even to unrelated organisms. Previously, several laboratories have shown that the antibiotic resistance genes of DT104 are chromosomally encoded and involve integrons. The antibiotic resistance genes conferring the ACSSuT-resistant phenotype have been cloned and sequenced. These genes are grouped within two district integrons and intervening plasmid-derived sequences. This sequence is potentially useful for detection of multiresistant DT104.

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Figures

FIG. 1
FIG. 1
Arrangement of antibiotic resistance genes of S. typhimurium DT104 isolate H3380 as deduced from the nucleic acid sequence described in this work. Black boxes represent 59-base elements.
FIG. 2
FIG. 2
Comparison of the chloramphenicol resistance gene product of an S. typhimurium DT104 isolate, H3380, to the most similar GenBank entry R10CMLA (PID: g151756); amino acids are indicated by their standard one-letter designations. The top row of sequence refers to the DT104 protein sequence. The bottom row is from a CmlA protein sequence of P. aeruginosa. The center line indicates amino acid identity when an amino acid is written, similarity when a plus symbol is present, and lack of similarity when nothing is written.
FIG. 3
FIG. 3
A representative result for the long PCR of S. typhimurium DT104 and non-DT104 isolates. Lane 1, MW markers; lanes 3, 5 to 7, and 12, (ACSSuT-resistant) DT104 (S3455, S2486, 13HP, H3380, and S2490, respectively); lanes 2, 4, 9, and 11, non-DT104 (F4797, S3426, S. hadar MF60404, and S3444, respectively); lanes 8 and 10, (ASSuT-resistant) DT104 (H3402 and S3461, respectively). A positive result is evidenced by the presence of a band of 10,036 bp.
See this image and copyright information in PMC

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