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. 1999 Apr;65(4):1455-8.
doi: 10.1128/AEM.65.4.1455-1458.1999.

Identification of Cryptosporidium felis in a cow by morphologic and molecular methods

F J Bornay-Llinares  1 , A J da Silva, I N Moura, P Myjak, H Pietkiewicz, W Kruminis-Lozowska, T K Graczyk, N J Pieniazek
Affiliations

Identification of Cryptosporidium felis in a cow by morphologic and molecular methods

F J Bornay-Llinares et al. Appl Environ Microbiol. 1999 Apr.

Abstract

Apicomplexan Cryptosporidium parasites infect a wide range of vertebrate hosts. While some species are limited to a single host group, such as Cryptosporidium baileyi, which infects chickens, other species of this genus, such as C. parvum, infect a wide range of mammalian species from mice to humans. During an investigation of Cryptosporidium infection in cattle on a farm in northern Poland, we identified an infection caused by C. felis, in addition to known infections with C. muris and C. parvum. This new infection was identified based on the size of the oocysts (mean size, 4.3 +/- 0.4 micrometer; range, 3.5 to 5.0 micrometer), as well as by analysis of the molecular sequence of the variable region of the small-subunit rRNA. This finding demonstrates the complex host specificity and circulation in the environment of Cryptosporidium species.

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Figures

FIG. 1
FIG. 1
PCR detection of Cryptosporidium-specific diagnostic fragments. Lanes: 1, cow isolate PM741; 2, cow isolate PM348; S, 50-bp ladder used as a DNA fragment size standard. The values beside the gel are DNA fragment sizes in base pairs. The expected C. muris-specific diagnostic band size is 431 bp, and the C. felis-specific fragment is 455 bp.
See this image and copyright information in PMC

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