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. 1978 Sep 1;173(3):877-83.
doi: 10.1042/bj1730877.

Immunochemical characterization of casein from rabbit mammary gland

K Al-Sarraj, D A White, R J Mayer

Immunochemical characterization of casein from rabbit mammary gland

K Al-Sarraj et al. Biochem J. .

Abstract

1. Excellent precipitating antibodies to rabbit recombined casein polypeptides were obtained in a sheep after 8 weeks of immunization with rabbit recombined polypeptides coupled to Sepharose-albumin. 2. The antiserum was assessed for specificity by several immunochemical techniques and was monospecific when tested against acid-precipitated casein, recombined casein and extracts of lactating rabbit mammary tissue. 3. A specific anti-casein immunoglobulin fraction was prepared by immunoadsorption of the antiserum by using Sepharose-recombined casein as immunoadsorbent. 4. The specific anti-casein immunoglobulin was used to prepare a Sepharose-anti-casein immunoadsorbent for the isolation of casein from extracts of rabbit mammary tissue.

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