Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Nov;3(2):143-50.
doi: 10.1007/BF00423228.

Molecular weights, poly(A)--content, and partial separation of chick globin mRNAs

W Knochel, D Lange, D Hendrick

Molecular weights, poly(A)--content, and partial separation of chick globin mRNAs

W Knochel et al. Mol Biol Rep. 1976 Nov.

Abstract

Poly(A)-containing 9S RNA from chick reticulocytes was electrophoresed on formamide-polyacrylamide gels. The molecular weight was determined to be 211 000 +/- 10 000 daltons. The RNA was separated into three different fractions with respect to molecular weight. These RNAs were translated in a wheat germ cell-free system. The lower molecular weight RNA directed up to 95% alpha-chain synthesis, compared to 60% for the higher molecular weight RNA. This was accompanied by a relative increase for beta-chain synthesis with increasing molecular weight. It could also be shown by hybridization with labelled poly(U) that the average poly(A) length decreased from about 83 nucleotides for fraction I to 36 nucleotides for fraction III. Our results suggest that fractionation of avian 9 S globin mRNA by electrophoresis on formamide-polyacrylamide gels is dependent upon two parameters, namely differences in the lengths of the non-poly(A)-containing portion of the alpha and beta mRNAs and differences in the poly(A) lengths.

PubMed Disclaimer

References

    1. Nat New Biol. 1972 Jan 26;235(56):108-10 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2716-9 - PubMed
    1. Nat New Biol. 1973 Aug 22;244(138):244 - PubMed
    1. Biochim Biophys Acta. 1974 Dec 18;371(2):379-91 - PubMed