The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization
- PMID: 101767
- DOI: 10.1007/BF00333153
The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization
Abstract
Transfer RNAAsp2delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated "in vitro" with Na [125I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAAsp2 to the left arm of the second chromosome in the regions 29 D and E.
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