doi: 10.1007/BF00333165.
Bacillus subtilis dnaF: a mutation of the gene specifying the structure of DNA polymerase III
- PMID: 101768
- DOI: 10.1007/BF00333165
Item in Clipboard
Bacillus subtilis dnaF: a mutation of the gene specifying the structure of DNA polymerase III
Mol Gen Genet.
.
Abstract
The characteristics of Bacillus subtilis dnaF, a mutation specifying a temperature sensitive phenotype, were examined to determine its relationship to polC, the gene specifying the structure of DNA polymerase III (pol III). Exposure of growing cells bearing dnaF to non-permissive temperature inhibited replicative DNA synthesis and specifically depressed the expression of pol III activity in crude extracts. Highly purified pol III derived from cells bearing dnaF was temperature.sensitive in its polymerase activity, indicating that dnaF is a specific, polC mutation which specifies a structurally altered enzyme.
Similar articles
-
On the identity of dnaP and dnaF genes of Bacillus subtilis.Mol Gen Genet. 1976 Oct 18;148(1):9-17. doi: 10.1007/BF00268540. Mol Gen Genet. 1976. PMID: 825719
-
Bacillus subtilis DNA polymerase III: complete sequence, overexpression, and characterization of the polC gene.Gene. 1991 Feb 1;98(1):29-36. doi: 10.1016/0378-1119(91)90100-p. Gene. 1991. PMID: 1901559
-
DNA polymerase III gene of Bacillus subtilis.Proc Natl Acad Sci U S A. 1989 Jun;86(12):4421-4. doi: 10.1073/pnas.86.12.4421. Proc Natl Acad Sci U S A. 1989. PMID: 2499883 Free PMC article.
-
Is there a role for replication fork asymmetry in the distribution of genes in bacterial genomes?Trends Microbiol. 2002 Sep;10(9):393-5. doi: 10.1016/s0966-842x(02)02420-4. Trends Microbiol. 2002. PMID: 12217498 Review.
-
A panoply of errors: polymerase proofreading domain mutations in cancer.Nat Rev Cancer. 2016 Feb;16(2):71-81. doi: 10.1038/nrc.2015.12. Nat Rev Cancer. 2016. PMID: 26822575 Review.
Cited by
-
Development of novel inhibitor probes of DNA polymerase III based on dGTP analogs of the HPUra type: base, nucleoside and nucleotide derivatives of N2-(3,4-dichlorobenzyl)guanine.Nucleic Acids Res. 1990 Dec 25;18(24):7381-7. doi: 10.1093/nar/18.24.7381. Nucleic Acids Res. 1990. PMID: 2259629 Free PMC article.
-
Purification and characterization of DNA polymerase alpha of Chinese hamster ovary cells.Mol Cell Biochem. 1985 Oct;68(2):169-79. doi: 10.1007/BF00219381. Mol Cell Biochem. 1985. PMID: 3001502
-
Genetic structure and domains of DNA polymerase III of Bacillus subtilis.Mol Gen Genet. 1991 May;226(3):467-72. doi: 10.1007/BF00260660. Mol Gen Genet. 1991. PMID: 1840638
-
Cloning and characterization of the polC region of Bacillus subtilis.J Bacteriol. 1986 Mar;165(3):951-7. doi: 10.1128/jb.165.3.951-957.1986. J Bacteriol. 1986. PMID: 2936731 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
