Testosterone stimulation of the medial preoptic area and medial amygdala in the control of male hamster sexual behavior: redundancy without amplification

Abstract

Receptors for gonadal steroids are present in an interconnected network of limbic nuclei. The existence of this network structure has important implications for how steroids control reproductive physiology and behavior. In 1986, Cottingham and Pfaff proposed that properties of a steroid-responsive neural network could include redundancy, amplification, stability and selective filtering. The present study tested the concept of steroid amplification, using male hamster sexual behavior as a model. In the male hamster, the medial amygdaloid nucleus (Me) and medial preoptic area (MPOA) are essential for mating behavior, and both nuclei transduce steroid cues to facilitate copulation. To determine if steroid action at multiple interconnected nuclei amplifies mating, the present study compared sexual behavior in castrated male hamsters bearing unilateral intracranial implants of testosterone in Me or MPOA with that of males with dual testosterone implants in Me and MPOA. Implants that stimulated androgen receptor-containing neurons in Me or MPOA stimulated copulatory behavior above castrate levels. However, behavior of males with dual implants was not significantly different from that of males with single implants. This suggests that steroid action at either MPOA or Me is sufficient to facilitate mating, but dual stimulation of these reciprocally-connected nuclei does not amplify sexual behavior.