Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1999 Apr;37(4):1229-30.
doi: 10.1128/JCM.37.4.1229-1230.1999.

Should genotypic testing be done on all phenotypically vancomycin- resistant enterococci detected in hospitals?

G W Coombs, I D Kay, R A Steven, J W Pearman, D Bertolatti, W B Grubb

Should genotypic testing be done on all phenotypically vancomycin- resistant enterococci detected in hospitals?

G W Coombs et al. J Clin Microbiol. 1999 Apr.
No abstract available

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Agarose gel electrophoresis of vanA, vanB, vanC-1, and vanC-2 PCR products. (A) Multiplex vanA and vanB gene PCR (lanes 2 to 7) and vanC-1 gene PCR (lanes 9 to 14). Lanes: 1 and 8, Amresco PCR DNA marker; 2, E. faecalis ATCC 51299 vanB, 297 bp (product size, 297 bp); 3, E. faecium wild strain vanA, 783 bp (product size, 783 bp); 4, E. gallinarum NCTC 11428 vanC-1, 822 bp (no product detected); 5, E. casseliflavus ATCC 25788 vanC-2, 439 bp (no product detected); 6, WBG 9213 (product size, 783 bp); 7, water control; 9, E. faecalis ATCC 51299 vanB, 297 bp (no product detected); 10, E. faecium wild strain vanA, 783 bp (no product detected); 11, E. gallinarum NCTC 11428 vanC-1, 822 bp (product size, 822 bp); 12, E. casseliflavus ATCC 25788 vanC-2, 439 bp (no product detected); 13, WBG 9213 (product size, 822 bp); 14, water control. (B) vanC-2 gene PCR. Lanes: 1, Amresco PCR DNA marker; 2, E. faecalis ATCC 51299 vanB, 297 bp (no product detected); 3, E. faecium wild strain vanA, 783 bp (no product detected); 4, E. gallinarum NCTC 11428 vanC-1, 822 bp (no product detected); 5, E. casseliflavus ATCC 25788 vanC-2, 439 bp (product size, 439 bp); 6, WBG 9213 (no product detected); 7, water control.
See this image and copyright information in PMC

References

    1. Centers for Disease Control and Prevention. Nosocomial enterococci resistant to vancomycin—United States, 1989–1993. Morbid Mortal Weekly Rev. 1993;42:597–599. - PubMed
    1. Dutka-Malen S, Blaimont B, Wauters G, Courvalin P. Emergence of high-level resistance to glycopeptides in Enterococcus gallinarum and Enterococcus casseliflavus. Antimicrob Agents Chemother. 1994;38:1675–1677. - PMC - PubMed
    1. Facklam R R, Collins M D. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol. 1989;27:731–734. - PMC - PubMed
    1. Free L, Sahm D. Detection of enterococcal vancomycin resistance by multiplex PCR. In: Persing D H, editor. PCR protocols for emerging infectious diseases. Washington, D.C: ASM Press; 1996. pp. 150–156.
    1. Leclercq R, Courvalin P. Resistance to glycopeptides in enterococci. Clin Infect Dis. 1997;24:545–556. - PubMed

MeSH terms