Binding of phenylbutazone to human serum albumin. Characterization and identification of binding sites

Abstract

The quantitative parameters characterizing the binding of phenylbutazone (CAS 50-33-9) to human serum albumin are determined using the circular dichroism titration method and a combined mathematical approach. The values of affinity constants obtained are in good agreement with those found in the literature. The characterization and identification of binding sites is also performed. A conclusion is derived that phenylbutazone can be used as a model compound for investigation of ligand-macromolecule interactions.