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. 1999 Apr 1;66(2):115-23.
doi: 10.1016/s0378-1135(99)00004-8.

Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

G M Allan  1 , F Mc Neilly, B M Meehan, S Kennedy, D P Mackie, J A Ellis, E G Clark, E Espuna, N Saubi, P Riera, A Bøtner, C E Charreyre
Affiliations

Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

G M Allan et al. Vet Microbiol. .

Abstract

A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).

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