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. 1999 Feb;96(2):215-9.
doi: 10.1046/j.1365-2567.1999.00693.x.

Inhibitory effect of interleukin-16 on interleukin-2 production by CD4+ T cells

H Ogasawara  1 , N Takeda-Hirokawa, I Sekigawa, H Hashimoto, Y Kaneko, S Hirose
Affiliations

Inhibitory effect of interleukin-16 on interleukin-2 production by CD4+ T cells

H Ogasawara et al. Immunology. 1999 Feb.

Abstract

Signalling through CD4 by human immunodeficiency virus (HIV)-1 envelope glycoprotein (gpl20) and/or anti-CD4 antibodies can promote T-cell activation and anergy. Interleukin (IL)-16 is a competence growth factor for CD4+ T cells that can induce a G0 to G1 cell cycle transition but cannot induce cell division. The receptor of this cytokine is thought to be the CD4 molecule, although the binding epitope of IL-16 differs from that of HIV. We have demonstrated that both HIV-1/gp120 and IL-16 induced CD4+ T-cell dysfunction, as indicated by suppression of mitogen-induced IL-2 production. Two anti-CD4 antibodies with different binding sites on CD4 also showed an inhibitory effect on IL-2 production. These results indicate that promotion of CD4+ T-cell anergy via the CD4 molecule does not depend on the binding sites of the CD4 ligands.

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Figures

Figure 1
Figure 1
Effect of HIV-1/gp120 on Con A-mediated IL-2 production. (○)+Con A; (•) −Con A. There was a significant difference in Con A-induced IL-2 production between cultures without HIV-1/gp120 and with HIV-1/gp120 (101 μg/ml) (P < 0·001).
Figure 2
Figure 2
Effect of two anti-CD4 antibodies, (a) a Leu-3a antibody and (b) an OKT4 antibody, on IL-2 production induced by Con A stimulation. (○)+Con A; (•) −Con A. There was a significant difference on Con A-induced IL-2 production between cultures without either antibody and cultures with Leu-3a (25 μg/ml) or OKT4 (50 μg/ml) (P < 0·01).
Figure 3
Figure 3
Effect of three anti-CD8 antibodies, (a) a Leu-2a antibody, (b) an OKT8 antibody, and (c) an anti-CD8β antibody, on the production of Con A-mediated IL-2 production. (○)+Con A; (•) −Con A. There were no significant differences in IL-2 production between cultures without and with any of these three antibodies.
Figure 4
Figure 4
Effect of IL-16 on IL-2 production induced by Con A. (○) +Con A; (•) −Con A. A significant difference in the IL-2 levels was observed between cultures without IL-16 and with IL-16 (105 pg/ml) (P < 0·001).
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