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. 1999 May 1;174(1):9-17.
doi: 10.1111/j.1574-6968.1999.tb13543.x.

Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

O Geisenberger  1 , A Ammendola, B B Christensen, S Molin, K H Schleifer, L Eberl
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Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

O Geisenberger et al. FEMS Microbiol Lett. .

Abstract

A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4 x 10(-6) to 1 x 10(-5) transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid.

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