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. 1976 Jul-Aug;12(4):572-7.

[Purification of pancreatic kallikrein by the method of affinity chromatography]

[Article in Russian]
V V Mosolov, E V Ievleva, V A Shaternikov, M F Samenkova
  • PMID: 1026941

[Purification of pancreatic kallikrein by the method of affinity chromatography]

[Article in Russian]
V V Mosolov et al. Prikl Biokhim Mikrobiol. 1976 Jul-Aug.

Abstract

Pancreatic Kallikrein was purified by affinity chromatography on BPTI-Sepharose. Immobilized BPTI was prepared via three stages: a) formation of the BPTI--acetylated trypsin complex; b) coupling of the resultant complex with CNBr-activated Sepharose; c) dissociation of the bound complex at pH 2.0 to yield immobilized BPTI and acetylated trypsin. The final product contained 59 X 10(-3) micronmoles insolubilized BPTI/ml Sepharose. Trypsin occurring in commercial Kallikrein preparations was separated by the batch procedure with ovomucoid-Sepharose. This method allows 100-fold purification of commercial Kallikrein.

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