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. 1976 May;2(3):271-80.
doi: 10.1007/BF01538965.

Polyethylene glycol-induced mammalian cell hybridization: effect of polyethylene glycol molecular weight and concentration

R L Davidson, K A O'Malley, T B Wheeler

Polyethylene glycol-induced mammalian cell hybridization: effect of polyethylene glycol molecular weight and concentration

R L Davidson et al. Somatic Cell Genet. 1976 May.

Abstract

The effects of polyethylene glycol (PEG) molecular weight and concentration on mammalian cell hybridization were studied. The peak hybridization-inducing activity with all grades of PEG from 400-6000 was found to occur in the concentration range of 50-55%. However, changes in concentration were seen to have different quantitative effects with different grades of PEG. For monolayer fusions, PEG 1000 at 50% seems to be the optimal combination of PEG molecular weight and concentration, in terms of both efficiency of hybridization and relative insensitivity to dilution effects.

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