[Functional characterization of the creatine phosphokinase reactions in heart mitochondria and myofibrils]
- PMID: 1030648
[Functional characterization of the creatine phosphokinase reactions in heart mitochondria and myofibrils]
Abstract
The kinetic properties of MM-isozyme of creatine phosphokinase (CPK) bound to heart myofibrils have been determined experimentally. It has been shown that CPK isozymes bound to the heart myofibrils and mitochondria are electrophoretically different, but have very similar kinetic properties. For both isozymes the ATP formation reaction is preferable. However, in heart mitochondria the kinetic properties of CPK are compensated for by a tight functional coupling with ATP-ADP translocase. Due to this coupling the ATP formed in the course of oxidative phosphorylation can be used completely for creatine phosphate production in mitochondria. On the other hand, the kinetic properties of myofibrillar CPK isozyme are such that they provide for the effective utilization of creatine phosphate produced in mitochondria for rephosphorylation of AKP formed in the myofibrils during contraction. It is concluded that in the heart cells energy can be transferred from the mitochondria to the myofibrils by creatine phosphate molecules.
Similar articles
-
[The functional coupling between MM isozyme of creatine phosphokinase (EC 2.7.3.2.) and MgATPase of myofibrils and (Na, K)ATPase of plasma membrane in heart cells].Biokhimiia. 1976 Dec;41(12):2099-109. Biokhimiia. 1976. PMID: 139170 Russian.
-
[A comparative study of the role of creatine phosphokinase isoenzymes in energy metabolism of skeletal and heart muscle].Biokhimiia. 1977 Apr;42(4):579-88. Biokhimiia. 1977. PMID: 870086 Russian.
-
Studies of energy transport in heart cells. Mitochondrial isoenzyme of creatine phosphokinase: kinetic properties and regulatory action of Mg2+ ions.Eur J Biochem. 1975 Sep 1;57(1):273-90. doi: 10.1111/j.1432-1033.1975.tb02299.x. Eur J Biochem. 1975. PMID: 126157
-
Role of creatine phosphokinase in cellular function and metabolism.Can J Physiol Pharmacol. 1978 Oct;56(5):691-706. doi: 10.1139/y78-113. Can J Physiol Pharmacol. 1978. PMID: 361188 Review.
-
[Creatine kinase isoenzymes--characterization and functions in cell].Postepy Biochem. 2008;54(3):274-83. Postepy Biochem. 2008. PMID: 19112826 Review. Polish.
Cited by
-
The role of endogenous catecholamine in the depressive effects of free fatty acids on isolated, perfused rat hearts.Basic Res Cardiol. 1978 Mar-Apr;73(2):208-19. doi: 10.1007/BF01906755. Basic Res Cardiol. 1978. PMID: 656025 No abstract available.
-
Protective effects of glucagon during the anaphylactic response in guinea-pig isolated heart.Br J Pharmacol. 1982 Jul;76(3):483-9. doi: 10.1111/j.1476-5381.1982.tb09243.x. Br J Pharmacol. 1982. PMID: 6179557 Free PMC article.