Control of the RIA method as viewed from the standpoint of the investigation on the kinetics of the insulin-125 I-antibody reaction

Abstract

In this paper attempts were made towards the optimalization and the control of some parameters of the RIA reaction. Basing on the law mass action, as well as on the Scatchard's and Sip's equations, the equilibrium constants for the reversible reaction: insulin-125I-antibody for different incubation temperatures were calculated. Moreover a characteristic of the antiinsulin antibody by means of the "alpha"heterogeneity coefficient was done, as well as the values of the thermodynamic function increments were calculated, which made possible to point out explicitly the optimal shape of the standard curve.