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. 1978 Nov;5(11):4025-38.
doi: 10.1093/nar/5.11.4025.

Formation and characterization of antibody against 2'-(5"-phosphoribosyl)-5' AMP, the monomer form of poly(adenosine diphosphate ribose)

H Sakura, M Miwa, Y Kanai, T Matsushima, T Sugimura
Free PMC article

Formation and characterization of antibody against 2'-(5"-phosphoribosyl)-5' AMP, the monomer form of poly(adenosine diphosphate ribose)

H Sakura et al. Nucleic Acids Res. 1978 Nov.
Free PMC article

Abstract

Specific antibody against 2'-(5"-phosphoribosyl)-5'AMP (PR-AMP), a monomer of poly(adenosine diphosphate ribose) (poly(ADP-Rib)), was produced by immunizing a rabbit with PR-AMP coupled to bovine serum albumin (BSA). Antibody against PR-AMP was purified 53-fold from serum by (NH4) 2SO4 precipitation, and BSA-Sepharose 4B, DEAE-cellulose and (PR-AMP)-BSA-Sepharose 4B column chromatographies. Inhibition experiments show that the adenine ring, 5'-phosphate residue and ribose-ribose bond of PR-AMP were essential for the antigenic determinant of PR-AMP. Anti PR-AMP antibody bound, not only with PR-AMP, but also with poly(ADP-Rib) of various chain lengths, while anti poly(ADP-Rib) antibody bound with poly(ADP-Rib) but not with PR-AMP.

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References

    1. Proc Natl Acad Sci U S A. 1964 Jul;52:68-74 - PubMed
    1. Biochemistry. 1977 May 17;16(10):2163-8 - PubMed
    1. Biochemistry. 1976 Mar 23;15(6):1213-7 - PubMed
    1. Rev Physiol Biochem Pharmacol. 1976;76:1-58, 177 - PubMed
    1. Biochemistry. 1977 Apr 5;16(7):1485-89 - PubMed