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Review
. 1999 Jun;37(6):1661-9.
doi: 10.1128/JCM.37.6.1661-1669.1999.

Principles and applications of methods for DNA-based typing of microbial organisms

D M Olive  1 , P Bean
Affiliations
Review

Principles and applications of methods for DNA-based typing of microbial organisms

D M Olive et al. J Clin Microbiol. 1999 Jun.
No abstract available

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Figures

FIG. 1
FIG. 1
Comparison of the procedural steps involved in various molecular typing methods. R.E., restriction endonuclease; O.N., overnight.
FIG. 2
FIG. 2
Equipment, reagents, and costs associated with molecular typing methods. Footnotes: 1, the large range in setup cost corresponds to the various types of automated DNA sequencing available; 2, material costs include enzymes, nucleotides, primers, disposable tubes and pipette tips, chemicals, purification kits, and electrophoresis reagents; 3, the material cost was calculated based on analysis of both DNA strands; 4, labor costs were calculated based on the total hands-on time of a technologist paid at a rate of $20.00/h and analyzing batches of 12 samples per run; 5, labor costs were calculated based on analysis of both DNA strands. rx, reaction.
See this image and copyright information in PMC

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