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Comparative Study
. 1999 Jun;37(6):1932-4.
doi: 10.1128/JCM.37.6.1932-1934.1999.

Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens

Affiliations
Comparative Study

Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens

S X Wang et al. J Clin Microbiol. 1999 Jun.

Abstract

Two hundred thirty respiratory specimens from 230 patients were analyzed by using COBAS AMPLICOR PCR, Amplified Mycobacterium tuberculosis Direct Test, and ligase chain reaction methods. Results were compared with those of smear microscopy and radiometric culture (Bactec) methods. No significant differences were observed among the results of the three methods, which are acceptable for direct detection of M. tuberculosis complex in respiratory specimens.

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References

    1. Abe C, Hirano K, Wada M, Kazumi Y, Takahashi M, Fukasawa Y, Yoshimura T, Miyagi C, Goto S. Detection of Mycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. J Clin Microbiol. 1993;31:3270–3274. - PMC - PubMed
    1. Beavis K G, Lichty M B, Jungkind D L, Giger O. Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens. J Clin Microbiol. 1995;33:2582–2586. - PMC - PubMed
    1. Bodmer T, Gurtner A, Schopfer K, Matter L. Screening of respiratory tract specimens for the presence of Mycobacterium tuberculosis by using the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test. J Clin Microbiol. 1994;32:1483–1487. - PMC - PubMed
    1. Carpentier E, Drouillard B, Dailloux M, Moinard D, Vallee E, Dutilh B, Maugein J, Bergogne-Berezin E, Carbonnelle B. Diagnosis of tuberculosis by Amplicor Mycobacterium tuberculosis test: a multicenter study. J Clin Microbiol. 1995;33:3106–3110. - PMC - PubMed
    1. D’Amato R F, Wallman A A, Hochstein L H, Colaninno P M, Scardamaglia M, Ardila E, Ghouri M, Kim K, Patel R C, Miller A. Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test. J Clin Microbiol. 1995;33:1832–1834. - PMC - PubMed

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