Typing of Salmonella enterica serotype paratyphi C isolates from various countries by plasmid profiles and pulsed-field gel electrophoresis

Abstract

Pulsed-field gel electrophoresis (PFGE) of 61 Salmonella enterica serotype Paratyphi C isolates from six countries gave five distinct clusters. Twenty-four isolates from five countries were susceptible to 10 antimicrobials tested and gave similar restriction endonuclease digest patterns of the 38-MDa plasmid. In contrast, plasmid and PFGE profiles of 37 multidrug-resistant isolates from Zaire were different from those from other countries.

FIG. 1

XbaI REFPs of representative Paratyphi C isolates from various countries. Lanes 1 and 18, 48.5-kb-ladder molecular size standard (sizes [in kilobases] are given to the left of the gel); lane 2, E48; lane 3, E49; lane 4, E51; lane 5, E52; lane 6, E53 in PFGE cluster 1; lane 7, E2 from Zaire in PFGE cluster 2; lane 8, E19 from Zaire in PFGE cluster 3; lane 9, E54 from Zaire in PFGE cluster 4; lane 10, E41 from Zaire in PFGE cluster 5; lane 11, E56 from Zaire in PFGE cluster 6; lane 12, E414 from India in PFGE cluster 7; lane 13, E1160 from India in PFGE cluster 3; lane 14, E1060 India in from PFGE cluster 3; lane 15, E701 from Iraq in PFGE cluster 8; lane 16, E699 from East Africa in PFGE cluster 3; lane 17, E700 from Greece in PFGE cluster 8. Strain types in lanes 2 to 6 were laboratory references from Zaire.