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. 1999 Jun;37(6):2077-9.
doi: 10.1128/JCM.37.6.2077-2079.1999.

Infection of laboratory mice with the human granulocytic ehrlichiosis agent does not induce antibodies to diagnostically significant Borrelia burgdorferi antigens

J E Bunnell  1 , L A Magnarelli, J S Dumler
Affiliations

Infection of laboratory mice with the human granulocytic ehrlichiosis agent does not induce antibodies to diagnostically significant Borrelia burgdorferi antigens

J E Bunnell et al. J Clin Microbiol. 1999 Jun.

Abstract

Laboratory diagnosis of Borrelia burgdorferi is routinely made by an enzyme-linked immunosorbent assay, with positive results confirmed by Western blot analysis. Concern has been raised that false-positive diagnoses may be made on the basis of serologic cross-reactivity with antibodies directed against other bacterial pathogens, in particular the agent of human granulocytic ehrlichiosis (HGE). The present study made use of a mouse model to ascertain the validity of these concerns. Two different strains of mice were inoculated with the HGE agent and assayed for production of polyclonal and monoclonal antibodies to antigens of both of these bacteria. Infection of mice with the HGE agent does not induce diagnostically significant B. burgdorferi serologic cross-reactions.

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References

    1. Bunnell J E, Dumler J S, Childs J E, Glass G E. Retrospective serosurvey for antibodies reactive to the HGE agent in Peromyscus leucopus from Baltimore, Maryland. J Wildl Dis. 1998;34:179–181. - PubMed
    1. Centers for Disease Control. Recommendations for test performance and interpretation from the Second National Conference on Serological Diagnosis of Lyme Disease. Morbid Mortal Weekly Rep. 1995;44:590–591. - PubMed
    1. Dumler J S, Asanovich K M, Bakken J S, Richter P, Kimsey R, Madigan J E. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic ehrlichia. J Clin Microbiol. 1995;33:1098–1103. - PMC - PubMed
    1. Edelman D C, Dumler J S. Evaluation of an improved PCR diagnostic assay for human granulocytic ehrlichiosis. Mol Diagn. 1996;1:41–49. - PubMed
    1. Hofmeister E K, Magera J, Sloan L, Kolbert C, Hanson J, Persing D H. Abstracts of the Seventh International Congress on Lyme Borreliosis, San Francisco, June 16–21. 1996. Borrelia burgdorferi proteins are recognized by antibodies from mice experimentally infected with the agent of human granulocytic ehrlichiosis, abstr. E814; p. 222.

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