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. 1976 Nov;9(3):431-8.
doi: 10.1016/0092-8674(76)90088-x.

Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit

N Maizels

Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit

N Maizels. Cell. 1976 Nov.

Abstract

Mapping with the restriction enzymes Sal 1 and R1 has generated a picture of the organization of Dictyostelium ribosomal DNA. The DNA which codes for 17S and 25S ribosomal RNAs is located within a stretch of repetitive DNA at least 38,000 base pairs long. This repeated unit includes 5S DNA, linked to 25S DNA. Two techniques were especially useful in the mapping: "cloning" 14S + 25S DNA on the plasmid pMB9 to amplify individual R1 fragments, and digesting DNA with R1 in the presence of the antibiotic distamycin A to produce specific partial digestion products.

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