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. 1976 Oct;14(4):1028-33.
doi: 10.1128/iai.14.4.1028-1033.1976.

Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus

T Honda, M Shimizu, Y Takeda, T Miwatani

Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus

T Honda et al. Infect Immun. 1976 Oct.

Abstract

A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column chromatographies on diethylaminoethylcellulose, diethylaminoethyl-Sephadex A-25, hydroxylapatite, and Sephadex G-200. This factor was separated from thermostabl direct hemolysin and was heat labile.

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