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. 1976 Nov;97(1):1-10.
doi: 10.1099/00221287-97-1-1.

Genetic analysis of radiation-sensitive mutations in the slime mould Dictyostelium discoideum

Genetic analysis of radiation-sensitive mutations in the slime mould Dictyostelium discoideum

D L Welker et al. J Gen Microbiol. 1976 Nov.

Abstract

The linkage of two mutations leading to increased sensitivity to ultraviolet light and 60Co gamma rays was determined in the slime mould Dictyostelium discoideum using a genetic analysis based on the parasexual cycle. Diploids were selected from a mixture of radiation-sensitive, temperature-resistant and radiation-resistant, temperature-sensitive haploids on the basis of simultaneous radiation and temperature resistance. Analysis of drug-resistant haploid segregants of the heterozygous diploids indicated that one of the radiation-sensitive mutations, radA20, was linked to linkage group I whereas the other, radB13, was linked to the recently defined linkage group VI.

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