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. 1976 Nov 5;366(2-3):195-202.
doi: 10.1007/BF00585878.

The influence of chloride on the ouabain-sensitive membrane potential and conductance of crayfish giant axons

The influence of chloride on the ouabain-sensitive membrane potential and conductance of crayfish giant axons

E M Lieberman et al. Pflugers Arch. .

Abstract

1. Resting potential and current-voltage relation were measured in crayfish giant axons bathed in chloride-free and sodium-free solutions with and without ouabain. 2. Chloride-free solution caused a transient depolarization but did not alter the steady-state membrane potential. Utilizing isethionate as an anion substitute, the membrane resistance increased 12.5%. 3. In the absence of extracellular chloride, ouabain (0.5-1 mM) depolarized the axon 6-7 mV. The shape of the current-voltage relation did not change but the curve was shifted along the current axis. 4. These results indicate that ouabain inhibits a steady-state hyperpolarizing electrogenic pump current of approximately 3 muA/cm2. 5. Extracellular sodium removal from axons equilibrated in chloride-free solutions transiently hyperpolarized the membrane 6-7 mV without a change in membrane resistance. The transient hyperpolarization was ouabain and temperature sensitive. The steady-state potential reached in sodium-free and chloride-free solution was not ouabain sensitive. Temperature sensitivity of the steady-state membrane potential was greatly reduced. 6. The transient hyperpolarization produced by extracellular sodium removal was metabolically driven and may present the expression of a sodium efflux transport current of 7.0-7.5 muA/cm2. 7. Using electrophysiologically measured parameters, sodium and potassium conductance, influx and efflux currents and the coupling ratio for sodium/potassium transport are calculated from a modification of the conductance equation. 8. The sodium/potassium transport coupling ratio for steady-state conditions was estimated at 5:3 (1.67:1).

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References

    1. J Gen Physiol. 1967 Aug;50(7):1835-48 - PubMed
    1. J Gen Physiol. 1968 Sep;52(3):408-23 - PubMed
    1. Acta Physiol Scand. 1967 Jul-Aug;70(3):431-48 - PubMed
    1. J Physiol. 1952 Apr;116(4):449-72 - PubMed
    1. J Gen Physiol. 1974 Dec;64(6):666-90 - PubMed

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