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Comparative Study
. 1976;52(3):207-16.
doi: 10.1007/BF01348018.

Plaque formation by African swine fever virus in chick embryo fibroblasts in the absence of CO2 atmosphere

C Gil-Fernandez, A García-Gancedo, P Vilas-Minondo
Comparative Study

Plaque formation by African swine fever virus in chick embryo fibroblasts in the absence of CO2 atmosphere

C Gil-Fernandez et al. Arch Virol. 1976.

Abstract

A plaque assay method using ASFV previously adapted to growth in chick embryo fibroblasts is described. Chick embryo fibroblast monolayers under bactoagar or methylcellulose have been employed using cysteine, arginine, DEAE-Dextran and HEPES as additives. Plaque production was optimal under methylcellulose. HEPES rendered the plaques more clear when used with the overlay. Arginine enhances plaque formation with bactoagar, and DEAE-Dextran doubles the plaque size. The growth curve of ASFV in chick embryo monolayers has been studied.

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References

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