[Determination of the biosynthetic chemotherapeutic index of antiviral agents in cell culture]
- PMID: 1034372
[Determination of the biosynthetic chemotherapeutic index of antiviral agents in cell culture]
Abstract
An experimental substantiation of an improved approach to testing antiviral chemotherapeutic agents in tissue culture was done on the model of Venezuelan equine encephalomyelitis virus and primarily trypsinized chick embryo fibroblasts using some biologically active agents such as actinomycin D, RNA-ase, gentamycin, poly (rI). poly (rC). The approach is based on combined use of stationary cultivation of cells and virlses, a method for determination of radioactivity of the cells grown directly in vials for scintillation counting as well as methods for determination of virus-specific and cell syntheses by incorporation of labeled precursors. The features of the mechanism of action of the agents under study is discussed in terms of their influence on cell metabolism.
Similar articles
-
[Study of the antiviral action of gentamicin].Antibiotiki. 1975 May;20(5):429-33. Antibiotiki. 1975. PMID: 1225192 Russian.
-
[Inhibition of multiplication of RNA-containing viruses in tissue culture by combined use of an interferon inducer and ribonuclease].Antibiotiki. 1973 Jul;18(7):629-33. Antibiotiki. 1973. PMID: 4359363 Russian. No abstract available.
-
[Reproduction of the Venezuelan equine encephalomyelitis virus under conditions of low ionic strength].Vopr Virusol. 1974 Sep-Oct;(5):586-75. Vopr Virusol. 1974. PMID: 4216162 Russian. No abstract available.
-
[Interrelationship of alphavirus-Venezuelan equine encephalomyelitis virus reproduction and oncornavirus type D production with the metabolism of the infected cells].Vopr Virusol. 1977;(1):50-4. Vopr Virusol. 1977. PMID: 910441 Russian.
-
[Antiviral action of nucleases and histones].Vopr Virusol. 1974 Nov-Dec;(6):647-52. Vopr Virusol. 1974. PMID: 4374831 Review. Russian. No abstract available.