The effect of different diets including those containing soya-bean products, on digesta movement and water and nitrogen absorption in the small intestine of the pre-ruminant calf

Abstract

1. Preruminant calves, fistulated in the abomassum and distal ileum, were given by infusion into the abomasum, at intervals of 2-3 d, single experimental feeds of cow's milk or a synthetic milk diet in which the protein source was casein or a soya-bean product. Polyethylene glycol (PEG) and phenol red were included as markers. 2. After infusion of the experimental feed, measurements were made of transit time from proximal duodenum to distal ileum and mean ileal flow-rates for the first 3 and 21 h after food residues reached the distal ileum. PEG recoveries for these two periods and net nitrogen absorption up to the distal ileum were also determined. In some experiments sodium, potassium and magnesium concentrations and numbers of viable bacteria in ileal digesta were determined. 3. Measurements of passage of digesta, recovery of marker and net N absorption did not differ greatly between calves given cow's milk and the synthetic milk diet containing casein. Compared to the casein-based diet, a diet based on heated soya-bean flour given to a calf for the first or second time produced a somewhat higher ileal flow-rate and a significantly lower net N absorption. Net N absorption was even lower when a calf received a diet containing unheated soya-bean flour. 4. Calves given the diet containing heated soya-bean flour on several occasions appeared to develop a sensitivity to the flour, and after further feeds containing it they had significantly higher rates of ileal flow (accompanied by correspondingly high rates of Na and K flow), lower small intestine transit times and lower net N absorption values than similarly fed unsensitized calves. They also had significantly lower rates of passage of marker in the first few hours after food residues reached the ileum, indicating inhibition of abomasal emptying. Over all, the changes indicated a severe disturbance in digestive function which was not caused by abnormal bacterial growth and may have been due to a gastrointestinal allergy. 5. Diets prepared from water-extracted flour and from soya-bean-protein isolate also led to digestive disturbances in sensitized calves, but those containing concentrates prepared by extracting soya-bean meal with hot aqueous ethanol did not.